Strongyloides IgG ELISA kit description:

The Strongyloides IgG ELISA Kit is an ELISA for the qualitative detection of IgG antibodies to Strongyloides stercoralis in serum and plasma.





Materials Provided with Strongyloides IgG ELISA Kit:
1. Strongyloides EIA Plate: containing Strongyloides 3 antigens
2. Enzyme Conjugate: Protein A conjugated to peroxidase
3. Strongyloides Elisa Kit Positive Control: diluted positive rabbit sera
4. Negative Control: diluted negative human serum
5. Chromogen: TMB
6. Strongyloides EIA Wash Concentrate 20X
7. Milk Dilution Buffer: buffered protein solution
8. Stop Solution: 11ml of 1 M phosphoric acid

Materials & Instrumentations required not included:
1. Precision pipettes
2. Distilled or deionized water
3. EIA kit Microplate Washer
4. EIA kit Microplate Reader with a 450 nm/620 - 650 nm filter





Strongyloides IgG ELISA Assay Kit Background Information
The parasite Strongyloides stercoralis is the causative agent for Strongyloidiasis disease. These parasites are endemic to tropical and semi-tropical environs, but are also found in various regions throughout the world. The parasite is known as an intestinal nematode which invades the body through exposed skin. Intestinal problems occur, such as diarrhea, but in more severe cases where the patients immune system may be compromised, meningitis or septic shock may develop. The Strongyloides ELISA kit is an especially appropriate serological test to use if the infection occurs outside the intestine where it is important to keep the disease isolated from other conditions, such as hemotologic malignancies.





Strongyloides IgG ELISA Test Principle
The principle of the Strongyloides ELISA test is a three-incubation process whereby the first incubation involves the coating of the wells with Strongyloides antigen. During this step, any antibodies that are reactive with the antigen, will bind to the coated wells. After wash enzyme conjugate is added. During this second incubation, the enzyme conjugate will bind to any antibodies present. Then, the chromogen TMB is added. With the presence of enzyme conjugate and the peroxidase causing the consumption of peroxide, the chromogen changes to a blue color. The blue color turns to a bright yellow color after the addition of the stop solution, which ends the reaction. For additional details please refer to the instructions for use.





Notable features of the Parasitology ELISA Kits:
User-friendly directions and explanation of test procedures
Simple and safe reagent preparation
Clear instructions on specimen collection
Comprehensive package of required materials
Explicit quality control and storage guidelines
Reliable and easy-to-read test results

Product inserts for most Parasitology ELISA kits follow a similar method. See the Strongyloides IgG ELISA Kit product insert for details on preparation, procedures, quality control, and test result interpretation.




Diagnostic Automation also provides other Parasitology ELISA Kits. For more information about ELISA Kits or Rapid Tests, IFA Kits, CLIA Test Kits, or Serology tests, please see our website home page, or contact our Customer Service Representative at 818-591-3030.