HSV 2 IgG CLIA kits - (Chemiluminescence Immuno Assay) description:

HSV 2 IgG Chemiluminescence ELISA is intended for use in evaluating a patient's serologic status to
herpes simples virus (HSV) infection, or for evaluating paired sera for the presence of a significant increase in herpes specific IgG.

Herpes Simplex Virus is a common pathogen and its primary infection is usually asymptomatic. There are
two immunologically distinct types of HSV: Type 1 and Type 2. HSV 1 is generally associated with oral
infection and lesions above the waist, and HSV 2 is associated with genital infections and lesions below the waist. Clinical cases primarily are 1) eczema herpeticum with eczematous skin changes with numerous lesions, 2) Gingivo-stomatitis and 3) Herpes sepsis, almost only found in newly born of premature infants. HSV IgG Chemiluminescence ELISA is an accurate serologic method to detect HSV specific antibody in serum sample.

Purified HSV antigen is coated on the surface of microwells. Diluted patient serum is added to wells, and
the HSV 2 IgG specific antibody, if present, binds to the antigen. All unbound materials are washed away. After adding enzyme conjugate, it binds to the antibody-antigen complex. Excess enzyme conjugate is washed off and substrate A & substrate B mixture is added. The light generated (RLU) is proportional to the amount of IgG specific antibody in the sample. The results are read by a microwell luminometer compared in a parallel manner with calibrator and controls.