Rheumatoid Factor IgM (RF) ELISA kit description:

The Diagnostic Automation Rheumatoid Factor (RF) ELISA kit is an enzyme-linked immunosorbent assay for the identification of IgM antibodies in human serum to RF antigen. The RF ELISA kit is intended for the detection of IgM antibodies in human serum to RF antigen and as an aid in the diagnosis of rheumatoid arthritis.




Materials provided with Rheumatoid Factor (RF) ELISA Test Kit:
1.Microplate: wells coated with Purified Rheumatoid Factor IgG antigen
2.Rheumatoid Factor EIA Test Serum Diluent Type II
3.Calibrator: Human serum or defibrinated plasma
4.Rheumatoid Factor ELISA Positive Control
5.Negative Control: Human serum or defibrinated plasma
6.Rheumatoid Factor Test Kit HRP Conjugate: Goat anti- human IgM
7.Chromogen/Substrate Solution Type I: TMB
8.Rheumatoid Factor EIA Test Kit Wash Buffer Type I: (20X concentrate)
9.Stop Solution: 1N H2SO4 solution

Materials Required, not Provided:
1. Precision pipettes
2. Distilled or deionized water
3. EIA kit Microplate Washer
4. EIA kit Microplate Reader with a 450 nm




RF ELISA Kit Background Information:
RFs are immunoglobulins of any isotype with antibody activity directed against antigenic sites on the Fc portion of IgG molecules. Because of its pentavalent structure and ability to cross-link immunoglobulin G antigen, IgM-RF is the main isotype identified by clinically available diagnostic assays for RF detection. Rheumatoid factors may exist as the mu, gamma, alpha, and epsilon isotypes. Rheumatoid factors are found in 1 to 4% of the general population. RFs are present in 75% of adult patients with the highest incidence of rheumatoid factors occurring in persons over 65 years of age and nearly all patients with Felty and Sjogren syndrome. The clinical correlation of an elevated rheumatoid factor should be interpreted cautiously. Increased titers may accompany a variety of acute immune responses, particularly viral infections and a number of other diseases infectious mononucleosis, tuberculosis, leprosy, various parasitic diseases, liver disease, sarcoidosis, and lymphoproliferative syndromes. The earliest tests and those still most widely used rely on the agglutinating properties of the IgM class of
rheumatoid factors, but enzyme immunoassays are more sensitive than agglutination and very specific due to use of purified antigen.



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RF ELISA Test Principle:
Diagnostic Automation Rheumatoid Factor test uses the ELISA technique for the detection of IgM antibodies to IgG antigen. The purified antigen is bound to a solid phase microassay well. Patient serum samples to be assayed for antibody are first diluted and added to each well. If antibody is present in the patient's serum, antigen-antibody complexes are formed. After washing the unbound serum from the well, horseradish peroxidase conjugated anti-human IgM is added to the wells and allowed to incubate. The conjugate will bind to human antibody which is present. For additional details please refer to the instructions for use.




Notable features of the Autoimmune Disease ELISA Kits:
. User-friendly directions and explanation of test procedures
. Simple and safe reagent preparation
. Clear instructions on specimen collection
. Comprehensive package of required materials
. Explicit quality control and storage guidelines
. Reliable and easy-to-read test results

Product inserts for most Autoimmune Disease ELISA kits follow a similar method. See the Rheumatoid Factor (RF) ELISA Kit product insert for details on preparation, procedures, quality control, and test result interpretation.

Diagnostic Automation/Cortez Diagnostics, Inc. also provides other Autoimmune Disease ELISA Kits. For more information about ELISA Kits or, Rapid Tests, IFA Kits, CLIA Test Kits, or Serology tests, please see our website home page, or contact our Customer Service Representatives at 818-591-3030 818-591-3030.