MPO (p-ANCA) ELISA kit
| Name |
MPO (p-ANCA) ELISA test |
|---|---|
| Full name |
Human MPO, Myeloperoxidase (p-ANCA) ELISA Test Kit |
| Category Name | Autoimmune Disease kits |
| Test | 96 |
| Method | ELISA method: Enzyme Linked Immunosorbent Assay |
| Principle | ELISA - Indirect; Antigen Coated Plate |
| Detection Range | Qualitative & Semi Quantitative Elisa assay: Positive, negative, cut off |
| Sample | 10 ul serum |
| Specificity | 97.90% |
| Sensitivity | 98.90% |
| Total Time | ~60 min |
| Shelf Life | 12 Months from the manufacturing date |
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MPO (p-ANCA) ELISA kit description:
The Diagnostic Automation Myeloperoxidase (MPO) ELISA kit is intended for the qualitative and semi-quantitative detection of IgG-class antibody to myeloperoxidase in human serum. This MPO ELISA test is intended to be used as an aid in the diagnosis of various autoimmune vasculitic disorders characterized by elevated levels of anti-neutrophil cytoplasmic antibodies (ANCA).
Material Provided with MPO (p-ANCA) IgG ELISA Kit:
1. Microplate: wells coated with inactivated Myeloperoxidase antigen
2. Conjugate:HRP goat anti-human IgG(Fc chain specific)
3. Positive Control: Human Serum
4. Calibrator: Human Serum
5. Negative Control: Human Serum
6. Sample Diluent
7. TMB
8. Stop Solution:1M H2SO4, 0.7M HCL
9. Wash Buffer Concentrate 10X
Materials Required, not Provided:
1. Precision pipettes
2. Distilled or deionized water
3. EIA kit Microplate Washer
4. EIA kit Microplate Reader with a 450 nm
MPO (p-ANCA) IgG ELISA Kit Background Information:
Antineutrophil cytoplasmic antibodies (ANCAs) are autoantibodies mainly IgG, directed against antigens found in the cytoplasmic granules of neutrophils and monocytes. Since their initial discovery, scientists have associated ANCA with a number of Systemic Vasculitides (SV). Two most common ANCAs are C-ANCA directed against Proteinase-3 (PR-3), and P-ANCA directed against Myeloperoxidase (MPO). ANCA testing is usually performed to help diagnose or exclude Wegener granulomatosis and microscopic polyangiitis. One of the most commonly used assay for ANCA is ELISA for ANCA both C-ANCA and P-ANCA.
MPO (p-ANCA) IgG ELISA Test Principle:
The Diagnostic Automation Myeloperoxidase (MPO) ELISA test system is designed to detect IgG class antibodies to MPO in human sera. Wells of plastic microwell strips are sensitized by passive absorption with MPO antigen. The Myeloperoxidase test procedure involves three incubation steps. For additional details please refer to the instructions for use.
Notable features of Autoimmune Disease ELISA Kits:
User-friendly directions and explanation of test procedures
Simple and safe reagent preparation
Clear instructions on specimen collection
Comprehensive package of required materials
Explicit quality control and storage guidelines
Reliable and easy-to-read test results
Product inserts for all Autoimmune ELISA kits follow a similar method. See the MPO (p-ANCA) IgG ELISA Kit product insert for specific details on preparation, procedures, quality control, and test result interpretation.
Diagnostic Automation Inc. also provides other Autoimmune Disease ELISA Kits. For more information about ELISA Kits, Rapid Tests, IFA Kits, CLIA Test Kits, or Serology tests, please see our website home page, or contact our Customer Service Representatives at 818-591-3030 .