SSA (Ro) ELISA kit
| Name |
SSA (Ro) ELISA Test |
|---|---|
| Full name |
Human SSA (Ro) ELISA Test Kit |
| Category Name | Autoimmune Disease kits |
| Test | 96 |
| Method | ELISA method: Enzyme Linked Immunosorbent Assay |
| Principle | ELISA principle- Peroxidase conjugated |
| Detection Range | Semi-Quantitative elisa assay- Positive, Negative and Cut-off |
| Sample | 10ul serum |
| Specificity | 100% |
| Sensitivity | 98.2% |
| Total Time | ~60 min |
| Shelf Life | 12 Months from the manufacturing date |
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SSA (Ro) ELISA kit description:
Diagnostic Automation SSA (Ro) ELISA test system is a semi-quantitative immunoassay for the detection of IgG antibodies to SSA (Ro) in human sera.
Material Provided with SSA (Ro) ELISA Kit:
1. Microplate: wells coated with inactivated antigen
2. Conjugate: HRP goat anti-human IgG
3. Positive Control: Human Serum
4. Calibrator: Human Serum
5. Negative Control: Human Serum
6. Sample Diluent
7. TMB
8. Stop Solution:1M H2SO4, 0.7M HCL
9. Wash Buffer Concentrate 10X
Materials Required, not Provided:
1. Precision pipettes
2. Distilled or deionized water
3. EIA kit Microplate Washer
4. EIA kit Microplate Reader with a 450 nm
SSA (Ro)ELISA Kit Background Information:
Autoantibodies directed against SSA and SSB may be observed in patients with SLE and Sjogren disease. SSA antibodies are frequently present in the serum of ANA negative SLE patients, such
as subacute cutaneous lupus erythematosus, a lupus-like syndrome associated with a homozygous C2 deficiency, and in a subset of patients who lack anti-dsDNA antibodies
SSA (Ro) ELISA Test Principle:
The Diagnostic Automation SSA (Ro) ELISA test kit is designed to detect IgG class antibodies to different autoantigens in human sera. Wells of plastic microwell strips are sensitized by passive absorption with immobilized antigens. The test procedure involves three incubation steps. For additional details please refer to the instructions for use.
Notable features of Autoimmune Disease ELISA Kits:
User-friendly directions and explanation of test procedures
Simple and safe reagent preparation
Clear instructions on specimen collection
Comprehensive package of required materials
Explicit quality control and storage guidelines
Reliable and easy-to-read test results
Product inserts for all Autoimmune ELISA kits follow a similar method. See the SSA (Ro) ELISA Kit product insert for specific details on preparation, procedures, quality control, and test result interpretation.
Diagnostic Automation Inc. also provides other Autoimmune Disease ELISA Kits. For more information about ELISA Kits, Rapid Tests, IFA Kits, CLIA Test Kits, or Serology tests, please see our website home page, or contact our Customer Service Representatives at 818-591-3030.