AFP ELISA kit description:

AFP ELISA test kit is an Enzyme Immunoassay test intended for the quantitative determination of AFP concentration in human serum.




Materials Provided with AFP ELISA Kit:
1. Microtiter wells coated with AFP ELISA Antibody
2. Zero buffer
3. Reference standard set: 0, 5, 20, 50, 150 & 300 ng/ml
4. AFP EIA Kit Enzyme Conjugate Reagent
5. TMB Substrate
6. AFP Elisa Test Stop Solution
7. Wash Buffer Concentrate 50X

Materials Required, not Provided:
1. Precision pipettes
2. Distilled or deionized water
3. EIA kit Microplate Washer
4. EIA kit Microplate Reader with a 450 nm




AFP ELISA Kit Background Information:
Elevation of serum AFP to abnormally high values, occurs in several malignant diseases, most notably non-seminomatous testicular cancer and primary hepatocellular carcinoma. In the case of non-seminomatous testicular cancer, a direct relationship has been observed between the incidence of elevated AFP levels and the stage of disease. Elevated AFP levels have also been observed in patients diagnosed with seminoma with non-seminomatous elements, but not in patients with pure seminoma. In addition, elevated serum AFP concentrations have been measured in patients with other noncancerous diseases, including ataxia telangiectasia, hereditary tyrosinemia, neonatal hyperbilirubinemia, acute viral hepatitis, chronic active hepatitis, and cirrhosis. Elevated serum AFP concentrations are also observed in pregnant women. Therefore, the AFP ELISA test kit measurements are not recommended for use as a screening procedure to detect the presence of cancer in the general population.

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AFP ELISA Test Principle:
The AFP ELISA kit is based on a solid phase enzyme-linked immunosorbent assay. The AFP ELISA kit utilizes one anti-AFP antibody for microtiter wells immobilization and another with mouse monoclonal anti-AFP antibody in the antibody-enzyme horseradish peroxidase conjugate solution. In the AFP ELISA kit, the serum is added to the AFP antibody coated microtiter wells and incubated with the Zero Buffer. If human AFP is present in the specimen, it will combine with the antibody on the well. For additional details please refer to the instructions for use.



Notable features of Cancer ELISA Kits:
User-friendly directions and explanation of test procedures
Simple and safe reagent preparation
Clear instructions on specimen collection
Comprehensive package of required materials
Explicit quality control and storage guidelines
Reliable and easy-to-read test results

Product inserts for all Cancer ELISA kits follow a similar method. See the AFP ELISA Kit product insert for specific details on preparation, procedures, quality control, and test result interpretation.

Diagnostic Automation Inc. also provides other Cancer ELISA Kits. For more information about ELISA Kits, Rapid Tests, IFA Kits, CLIA Test Kits, or Serology tests, please see our website home page, or contact our Customer Service Representatives at 818-591-3030 818-591-3030.