CA-15-3 ELISA kit
| Name |
CA-15-3 ELISA kit |
|---|---|
| Full name |
Human CA-15-3 ELISA Test Kit |
| Category Name | Cancer ELISA kits |
| Test | 96 |
| Method | ELISA method: Enzyme Linked Immunosorbent Assay |
| Principle | Sandwich Complex |
| Detection Range | 5-200U/mL |
| Sample | 100ul serum |
| Specificity | 97.5% |
| Sensitivity | 5 U/mL |
| Total Time | ~140 min |
| Shelf Life | 12-14 Months from the manufacturing date |
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CA-15-3 ELISA kit description:
The CA-15-3 EIA test is an ELISA assay for the quantitative measurement of CA-15-3 in human serum. CA153 elisa kit is intended for use as a monitoring and screening test for breast cancer.
Material Provided with CA153ELISA Kit:
1. Microtiter wells coated with Monoclonal Anti- CA-15-3 antibody
2. CA 15-3 EIA Test Sample diluent
3. CA 15-3 EIA Enzyme conjugate reagent
4. CA15-3 Elisa Test reference standards
5. TMB Substrate
6. CA 15-3 EIA Kit Stop solution
7. 50XWash Buffer Concentrate
Materials Required, not Provided:
1. Precision pipettes
2. Distilled or deionized water
3. EIA kit Microplate Washer
4. EIA kit Microplate Reader with a 450 nm
CA153ELISA Kit Background Information:
Breast cancer is the most common life-threatening malignant lesion in women of many developed countries today. 96% of patients with local and systemic recurrence have elevated CA15-3, which can be used to predict recurrence earlier than radiological and clinical criteria. A 25% increase in the serum CA15-3 is associated with progression of carcinoma. A 50% decrease in serum CA15-3 is associated with response to treatment. CA15-3 are more sensitive than CEA in early detection of breast cancer recurrence. In combination with CA-125, CA15-3 has been shown to be useful in early detection of relapse of ovarian cancer. CA15-3 levels are also increased in colon, lung and hepatic tumors. CA15-3 is most useful for monitoring patients post-operatively for recurrence, particularly metastatic diseases.
CA153ELISA Test Principle:
The CA15-3 EIA test is a two-site solid phase enzyme immunoassay. The molecules of CA15-3 are sandwiched between two monoclonal antibodies. One coated to the bottom of the wells of the microtiter plates and the other linked to the horseradish peroxidase enzyme conjugate. After incubation and washing, the enzymatic reaction develops a color which is proportional to the
amount of CA15-3 molecules present in the assay. For additional details please refer to the instructions for use.
Notable features of Cancer ELISA Kits:
User-friendly directions and explanation of test procedures
Simple and safe reagent preparation
Clear instructions on specimen collection
Comprehensive package of required materials
Explicit quality control and storage guidelines
Reliable and easy-to-read test results
Product inserts for all Cancer ELISA kits follow a similar method. See the CA153 ELISA Kit product insert for specific details on preparation, procedures, quality control, and test result interpretation.
Diagnostic Automation Inc. also provides other Autoimmune Disease ELISA Kits. For more information about ELISA Kits, Rapid Tests, IFA Kits, CLIA Test Kits, or Serology tests, please see our website home page, or contact our Customer Service Representatives at 818-591-3030 .