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Vitamin B12 ELISA kit


Vitamin B12 ELISA Test

Full name

Human Vitamin B12 ELISA Test kit

Category Name Anemia ELISA kits
Test 96
Method ELISA method: Enzyme Linked Immunosorbent Assay
Principle ELISA delay competitive
Sample 50 ul serum / plasma
Sensitivity 70.13 pg/ml
Total Time ~ 95 min
Shelf Life 12 Months from the manufacturing date

Item #:                    3125-15   Quantity:               

Vitamin B12 ELISA kit

Vitamin B12 3125-15 MSDS(08-12-2014).pdf

Vitamin B12 ELISA kit

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Vitamin B12 ELISA kit description:

The Diagnostic Automation, Inc. Vitamin B12 ELISA Kit is an enzyme immunoassay (ELISA) kit for the quantitative detection of Vitamin B12 concentration in human serum.

Materials provided with Vitamin B12 ELISA Test Kit:
1. Standard set for Vitamin B12 elisa test: 0, 100, 200, 400, 1000 & 2000 pg/ml
2. Vitamin B12 EIA enzyme conjugate reagent
3. Vitamin B12 EIA Biotin reagent
4. Streptavidin Coated Microplate 96 wells
5. Vitamin B12 EIA wash solution Concentrate
6. TMB substrate
7. Stop solution
8. Vitamin B12 ELISA releasing agent
9. Vitamin B12 ELISA stabilizing agent
10. Vitamin B12 ELISA neutralizing Buffer

Materials required but not provided:
1. Precision pipettes: 0.05~0.2ml
2. EIA kit Microplate Washer
3. EIA kit Microplate Reader at 450nm & 620nm wavelength
4. Quality control materials

Vitamin B12 ELISA Test Background Information:
Vitamin B12 is one of the 13 vitamins essential to human health and life. The essential Vitamin B12 is significant in the synthesis of DNA and the production of red blood cells. A healthy nervous system is maintained with Vitamin B12. The lack of Vitamin B12 damages the brain, spinal cord, nerves of the eye, and peripheral nerves. A B12 deficiency can result in symptoms ranging from severe anemia requiring blood transfusions, fatigue, muscle soreness, memory loss, and behavioral changes to serious and permanent nerve damage. B12 is naturally present in animal foods. Vitamin B12 can be received through supplements, and must follow a complex pathway of several steps for proper absorption in the body. Vitamin B12 plays an important role in the functioning of a healthy body. It is essential to the production of red blood cells that carry oxygen through the body's tissue. This in turn, helps in the development of myelination for proper functioning of the nervous system. The effects of Vitamin B12 deficiency can range from fatigue, weakness, depression, and memory problems to more severe symptoms such as anemia. Vitamin B12 deficiencies progress slowly, and thus it may take several years for initial signs to develop. Two groups of individuals that are most prone to Vitamin B12 deficiency are the elderly and vegans. Older adults lose the efficacy of the ability to absorb and digest water soluble vitamins (like Vitamin B12), and if there is not an efficient source of Vitamin B12 in the diet, they are at risk for a deficiency. Unless vegans take a regular supplement of Vitamin B12, they are prone to deficiency because their diet is void of one of the major sources of Vitamin B12 - animal meat. The absorption of Vitamin B12 occurs through saliva at the ingestion stage, and then during digestion, the B12 from food proteins is released with the help of acids in the system. The absorption of B12 depends on a protein called intrinsic factor (IF). Once combined with IF, Vitamin B12 can be absorbed in the intestines. Vitamin B12 and Folate deficiencies have similar symptoms, thus, it is important to have an accurate ELISA kit that can detect the differences. Even though the symptoms of both deficiencies show increased levels of homocysteine, a Vitamin B12 deficiency is the only one of the two that causes an additional increase in methylmalonyl CoA. Severe diseases that affect the vascular system; Parkinson's Disease, atherosclerosis, or coronary heart disease, are caused by increased levels of methylmalonyl CoA and homocysteines. This increase also causes stress at the cellular level, eventually leading to apoptosis.

Vitamin B12 ELISA Test Principle:
The essential reagents required for a enzyme immunoassay include antibody, enzyme-antigen conjugate and native antigen. Upon mixing the biotinylated antibody with a serum containing the antigen, a reaction results between the antigen and the antibody. After a short incubation, the enzyme conjugate is added (This delayed addition permits and increase in sensitivity for low concentration samples). Upon the addition of the enzyme conjugate, competition reaction results between the enzyme analog and the antigen in the sample for a limited number of antibody binging sites (not consumed in the first incubation). A simultaneous reaction between the biotin attached to the antibody and the streptavidin immobilized on the microwell occurs. This effects the separation of the antibody bound fraction after decantation or aspiration. This Vitamin B12 ELISA test kit can be run on an ELISA reader such as the Diagnostic Automation DAR800 ELISA testing instrument. The Diagnostic Automation, Inc. Vitamin B12 ELISA kits are designed for use in a laboratory, hospital lab or clinical lab setting.

For more information about ELISA Kits, Rapid Tests, IFA Kits, CLIA Test Kits, or Serology tests, please see our website home page, or contact our Customer Service Representative at 818-591-3030

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