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Human dsDNA ELISA Test Kit

Category Name Autoimmune Disease kits
Test 96
Method ELISA method: Enzyme Linked Immunosorbent Assay
Principle ELISA principle- Peroxidase conjugated
Detection Range Semi-Quantitative Elisa assay - Positive, Negative and Cut-off
Sample 10ul serum
Specificity 97.9%
Sensitivity 100%
Total Time ~ 65 min
Shelf Life 12-14 Months from the manufacturing date

Item #:                    2553-P1   Quantity:               




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dsDNA ELISA kit description:

dsDNA ELISA is intended for the detection of antibodies in human serum to dsDNA antigen and as an aid in diagnosis of Systemic lupus eruthematosus (SLE).

Material Provided with dsDNA ELISA Kit:
1. Microplate: wells coated with Purified dsDNA antigen
2. Serum Diluent Type II
3. Positive Control: Human serum or defibrinated plasma
4. Calibrator: Human serum or defibrinated plasma
5. Negative Control: Human serum or defibrinated plasma
6. HRP Conjugate: Goat anti-human IgG and IgM
7. Wash Buffer Type I 20X
8. Chromogen/Substrate Solution Type I
9. Stop Solution: 1N H2SO4 solution

Materials Required, not Provided:
1. Precision pipettes
2. Distilled or deionized water
3. EIA kit Microplate Washer
4. EIA kit Microplate Reader with a (single wavelength 450 nm or dual 600-650)


dsDNA ELISA Kit Background Information:
Autoimmune diseases are categorized into two general groups: One group is characterized by the production of tissue-specific autoantibodies, the other by autoimmune reactivity with normal cell nuclear and/or cytoplasmic antigens with no tissue type specificity. The latter group includes diseases such as mixed connective tissue disease, scleroderma, rheumatoid arthritis, dermatomyositis, polymyositis, SLE and Sjogren syndrome. ELISA test for dsDNA antibodies has many advantages over the immunofluorescent method including a sensitive, specific assay in an easy to use format providing quick and efficient results. The dsDNA antigen used in this kit is purified through modified immunoaffinity adsorption methods utilizing immobilized antigen-specific human immunoglobulins.

dsDNA ELISA Test Principle:
dsDNA elisa test is an Enzyme-Linked Immunosorbent Assay to detect antibodies to dsDNA antigen. Purified dsDNA antigen is attached to the wells. When antigens bound to the solid phase are brought into contact with a patient serum, antigen specific antibody, if present, will bind to the antigen in the wells forming antigen-antibody complexes. Excess antibody is removed by washing and followed by the addition of goat anti-human IgG, M conjugated with horseradish peroxidase which then binds to the antibody-antigen complexes. For additional details please refer to the instructions for use.

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Notable features of Autoimmune Disease ELISA Kits:
User-friendly directions and explanation of test procedures
Simple and safe reagent preparation
Clear instructions on specimen collection
Comprehensive package of required materials
Explicit quality control and storage guidelines
Reliable and easy-to-read test results

Product inserts for all Autoimmune ELISA kits follow a similar method. See the dsDNA ELISA Kit product insert for specific details on preparation, procedures, quality control, and test result interpretation.

Diagnostic Automation Inc. also provides other Autoimmune Disease ELISA Kits. For more information about ELISA Kits, Rapid Tests, IFA Kits, CLIA Test Kits, or Serology tests, please see our website home page, or contact our Customer Service Representatives at 818-591-3030 .