Sm/RNP ELISA kit
| Name |
Sm/RNP ELISA Test |
|---|---|
| Full name |
Human Sm/RNP ELISA Test Kit |
| Category Name | Autoimmune Disease kits |
| Test | 96 |
| Method | ELISA method: Enzyme Linked Immunosorbent Assay |
| Principle | Sandwich Complex |
| Detection Range | Semi-Quantitative elisa assay- Positive, Negative and Cut-off |
| Sample | 10ul serum |
| Specificity | 97.9% |
| Sensitivity | 92% |
| Total Time | ~60 min |
| Shelf Life | 20 Months from the manufacturing date |
 |
 |
 |
Sm/RNP ELISA kit description:
Diagnostic Automation Sm/RNP ELISA test kit is a semi-quantitative immunoassay for the detection of IgG antibodies to Sm/RNP in human sera. The result may aid in the diagnosis and treatment of autoimmune connective tissue disorders.
Materials provided with the Sm/RNP ELISA test kit:
1. Plate: 96 wells microplate coated with inactivated antigen
2. HRP-Conjugate: Conjugated HRP-goat anti-human IgG (Fc chain specific)
3. Positive Control: Human Serum
4. Calibrator: Human Serum
5. Negative Control: Human Serum
6. Sample Diluent
7. TMB
8. Stop Solution
9. Wash Buffer Concentrate (10X)
Materials required but not provided:
1. Freshly distilled or deionized water
2. Dispensing system and/or pipette
3. Microshaker for dissolving and mixing conjugate with samples
4. EIA kit Microplate washer
5. EIA kit Microplate Reader with 450nm wavelength
Sm/RNP ELISA Test Background Information:
In recent years, it has become clear that autoantibodies to a number of nuclear constituents have proven to be useful in the diagnosis of various connective tissue diseases.The presence of high level RNP antibodies alone are considered diagnostic of mixed connective tissue disease (MCTD) and are usually associated with a more benign disease course, while patients with low levels of RNP antibodies, together with other autoantibodies, may be observed in the serum of patients with progressive systemic sclerosis, Sjogren s Syndrome, and rheumatoid arthritis. The presence of RNP antibodies in the serum of SLE patients is usually associated with a lower incidence of renal involvement and a more benign disease course. To the contrary, patients with Sm antibodies experience a higher frequency of renal and central nervous system complications.
Sm/RNP ELISA Test Principle:
The Sm/RNP ELISA test kit is designed to detect IgG class antibodies to different autoantigens in human sera. Wells of plastic microwell strips are sensitized by passive absorption with immobilized antigens. The test procedure involves three incubation steps.
Notable features of Autoimmune Disease ELISA Kits:
User-friendly directions and explanation of test procedures
Simple and safe reagent preparation
Clear instructions on specimen collection
Comprehensive package of required materials
Explicit quality control and storage guidelines
Reliable and easy-to-read test results
Product inserts for most Autoimmune Disease ELISA kits follow a similar method. See the Sm/RNP ELISA Kit product insert for details on preparation, procedures, quality control, and test result interpretation.
For more information about ELISA Kits, Rapid Tests, IFA Kits, CLIA Test Kits, or Serology tests, please see our website home page, or contact our Customer Service Representative at 818-591-3030.
