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Human CEA ELISA Test Kit

Category Name Cancer ELISA kits
Test 96
Method ELISA method: Enzyme Linked Immunosorbent Assay
Principle Sandwich Complex
Detection Range 0-120 ng/mL
Sample 50ul serum
Specificity 95%
Sensitivity 1.0 ng/mL
Total Time ~80min
Shelf Life 12 Months from the manufacturing date

Item #:                    5201-16   Quantity:               


CEA_5201-16 (MSDS) 05-13-2016).pdf


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CEA ELISA kit description:

CEA ELISA test kit is an enzyme immunoassay intended for the quantitative determination of CEA concentration in human serum.

Material Provided with CEA ELISA Kit:
1. Microtiter wells coated with Antibody
2. 2. CEA standards containing; 0, 3, 12, 30, 60, and 120 ng/ml
3. Enzyme Conjugate Reagent
4. Substrate TMB
5. Stop Solution
6. Wash Buffer Concentrate 50X)
7. Control set (optional)

Materials Required, not Provided:
1. Precision pipettes
2. Distilled or deionized water
3. EIA kit Microplate Washer
4. EIA kit Microplate Reader with a 450 nm

CEA ELISA Kit Background Information:
Carcinoembroyonic antigen (CEA) is a cell-surface 200-kd glycoprotein. Increased levels of CEA are observed in more than 30% of patients with cancer of the lung, liver, pancreas, breast, colon, head or neck, bladder, cervix, and prostate. Elevated plasma levels are related to the stage and extent of the disease, the degree of differentiation of the tumor, and the site of metastasis. CEA is also found in normal tissue.

CEA ELISA Test Principle:
The CEA EIA test is a solid phase two-site immunoassay. The molecules of CEA are sandwiched between two monoclonal antibodies. One coated to the bottom of the wells of the microtiter plates and the other linked to the horseradish peroxidase enzyme conjugate. After incubation and washing, the enzymatic reaction develops a color which is proportional to the
amount of CEA molecules present in the assay. For additional details please refer to the instructions for use.

Notable features of Cancer ELISA Kits:
User-friendly directions and explanation of test procedures
Simple and safe reagent preparation
Clear instructions on specimen collection
Comprehensive package of required materials
Explicit quality control and storage guidelines
Reliable and easy-to-read test results
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Product inserts for all Cancer ELISA kits follow a similar method. See the CEA ELISA Kit product insert for specific details on preparation, procedures, quality control, and test result interpretation.

Diagnostic Automation Inc. also provides other Autoimmune Disease ELISA Kits. For more information about ELISA Kits, Rapid Tests, IFA Kits, CLIA Test Kits, or Serology tests, please see our website home page, or contact our Customer Service Representatives at 818-591-3030 .