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Human PAPP-A ELISA Test Kit

Category Name Fertility ELISA kits
Test 96
Method ELISA method: Enzyme Linked Immunosorbent Assay
Principle Sandwich Complex
Detection Range 0 - 30 ug/mL
Sample 10 uL serum/plasma
Specificity 97%
Sensitivity 0.133 ug/ml
Total Time ~75 min
Shelf Life 12-14 months from the manufacturing date

Item #:                    4229-6   Quantity:               


Papp-A 4229-6 MSDS(05-13-2016).pdf


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PAPP-A ELISA kit description:

The Diagnostic Automation PAPP-A ELISA is an enzyme immunoassay for the quantitative in vitro diagnostic measurement of Pregnancy associated plasma protein A (PAPP-A) in human serum and plasma.

Material Provided with PAPP-A ELISA Kit:
1. Microtiterwells: wells coated with anti-PAPP-A antibody (polyclonal)
2. Standards: Concentrations: 0; 1; 2.5; 5.0; 15.0; 30.0 ug/mL
3. Controls: (low and high)
4. Assay Buffer
5. Enzyme Conjugate 11X concentrate
6. Conjugate Diluent
7. Substrate Solution
8. Stop Solution

Materials Required, not Provided:
1. Precision pipettes
2. Distilled or deionized water
3. EIA kit Microplate Washer
4. EIA kit Microplate Reader with a 450 nm

PAPP-A ELISA Kit Background Information:
PAPP-A is a protein produced by the developing placenta Its concentration in the maternal blood increases rapidly after the 7th week of pregnancy. The measurement of PAPP-A in the first trimester of pregnancy has been reported as a useful marker in antenatal screening for Down Syndrome and other fetal aneuploidies. Reduced PAPP-A values in combination with maternal age, the measurement of free Beta-HCG and the ultrasonic determination of nuchal translucency in pregnancy weeks 11 to 14 may detect up to 90 % of pregnancies with Down syndrome. Diagnostic Automation PAPP-A ELISA EIA-2397 may be used for the risk assessment of Down's syndrome (trisomy 21) in the first trimester of pregnancy.

PAPP-A ELISA Test Principle:
This PAPP-A ELISA Kit is a solid phase ELISA based on the sandwich principle. The microtiter wells are coated with a polyclonal anti PAPP-A antibody. An aliquot of patient sample containing endogenous PAPP-A is incubated in the coated well with assay buffer. After incubation the unbound material is washed off. In the second incubation step a sandwich complex is formed with a polyclonal anti PAPP-A antibody peroxidase conjugate. Having added the substrate solution, the intensity of color developed is proportional to the concentration of PAPP-A in the patient sample. For additional details please refer to the instructions for use.

Diagnostic Automation Inc. also provides other Fertility ELISA Kits. For more information about ELISA Kits, Rapid Tests, IFA Kits, CLIA Test Kits, or Serology tests, please see our website home page, or contact our Customer Service Representatives at 818-591-3030 .

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