Folate ELISA Kit description:

Folate-Folic acid in food Elisa test kit provides an Enzyme Immunoassay for the quantitative determination of Folic Acid in food.




Materials Provided with Folate Elisa Kit:
1. Microtiter plate:12 strips with 8 breakable wells coated with folic acid conjugate
2. Folic Acid Standards (0; 4; 10; 40; 100; 400 ng/mL): 6 vials with 1.0 mL each
3. Anti-Folic Acid Antibody (mouse): 6 mL
4. Conjugate (anti-mouse-IgG-HRP): 15 mL
5. Substrate Solution (TMB): 15 mL
6. Stop Solution (0.5 M H2SO4): 15 mL
7. Sample diluent (PBS) 2x
8. Washing Solution (PBS + Tween 20): 60 mL as 10x
9. Two plastic foils to cover the strips during the incubation
10. Plastic bag to store unused microtiter strips
11. Instruction Manual

Materials required but not provided:
1. Freshly distilled or deionized water
2. Dispensing system and/or pipette
3. EIA kit Microplate washer
4. EIA kit Microplate Reader with 450nm wavelength




Folic Acid ELISA Kit Background Information:
Folates play an important role in the synthesis of nucleic acids and some amino acids and gained recently increased interest because they belong to the group of antioxidative vitamins. In the last years the influence of folic acid supplementation to avoid abortion and dysraphism was a topic of research increasingly. Folic acid as the most stable representative of the group of folates is added to a broad range of food. Traditional methods are mostly microbiological ones, but also TLC and HPLC are applied. These methods are time consuming and need complex equipment. This ELISA test kit allows the detection :2.5 to 4 hrs, incl. sample preparation- of folic acid in supplemented food which is more rapid compared to traditional techniques :24-48 hrs.



Folate ELISA Test Principle:
The Diagnostic Automation Folic Acid quantitative ELISA test is based on the principle of the enzyme linked immunosorbent assay-ELISA method. A folic acid conjugate is bound on the surface of a microtiter plate. Folic acid containing samples or standards and an antibody directed against folic acid are given into the wells of the microtiter plate. Immobilized and free folic acid compete for the antibody binding sites



Diagnostic Automation Inc. offers several popular Food ELISA Test Kits: Fumonisin ELISA Kit, Streptomycin ELISA Kit, Aflatoxin M1 ELISA Kit, Penicillin ELISA Kit, Histamine ELISA Kit, and Verotoxin Antigen in Food ELISA Test Kit. For more information on any of these Food ELISA Test Kits please see our website home page, or contact our Customer Service Representative at 818-591-3030.