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Peanut ELISA kit


Peanut ELISA Test

Full name

Food Peanut ELISA Test Kit

Category Name Food ELISA kits
Test 96
Method ELISA method: Enzyme Linked Immunosorbent Assay
Principle ELISA principle- Peroxidase conjugated
Detection Range Qualitative elisa assay- Positive, Negative Controls
Sensitivity 0.1 ppm
Total Time 60 min

Item #:                    5143-8   Quantity:               

Peanut ELISA kit


Peanut ELISA kit

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Peanut ELISA kit description:

Diagnostic Automation Inc. offers a Peanut ELISA Kit that is an Enzyme Immunosorbent Assay for the Qualitative Determination of Peanut in food. This Peanut ELISA Kit is a rapid and sensitive Food Allergen ELISA Kit which is designed to provide a sensitive and convenient method for screening food samples for peanut residues. The Peanut ELISA Kit represents a highly sensitive detection system and is particularly capable of the quantification of peanut residues in cookies, cereals, ice cream, and chocolate.

Materials Provided with Peanut ELISA Test Kit:
1. Microtiter plate: 12 strips with 8 breakable wells coated with anti-peanut antibodies
2. Peanut Standards (0; 1; 4; 10; 40 ppm of peanut): 5 vials with 1.0 mL each
3. Conjugate (anti-peanut-peroxidase): 15 mL
4. Substrate Solution (TMB): 15 mL
5. Stop Solution (0.5 M H2SO4): 15 mL
6. Extraction and sample dilution buffer (Tris): 2 x
7. Washing Solution (PBS + Tween 20): 60 mL as 10x
8. Plastic bag to store unused microtiter strips.
9. Instruction Manual

Materials required but not provided:
1. Freshly distilled or deionized water
2. Dispensing system and/or pipette
3. EIA kit Microplate washer
4. EIA kit Microplate Reader with 450nm wavelength

Peanut ELISA Test Kit Background Information:
Peanut (Arachis hypogaea) belongs to the legumes. With 25 %, the fraction of proteins in peanuts is very high. Many of these proteins are known for being allergenic, such as Arachins and Conarachins which are contained in relative high amounts. For this reason peanut represents one of the most important food allergens. For peanut allergic persons, hidden peanut allergens in food are a critical problem. Already very low amounts of peanuts can cause allergic reactions, which may lead to anaphylactic shock in severe cases. Because of this, peanut allergic persons must strictly avoid the consumption of peanuts or peanut containing food. Cross-contamination, mostly in consequence of the production process is often noticed. The chocolate production process is a representative example. This explains why in many cases the existence of peanut residues in foods cannot be excluded. Thus, sensitive detection systems, like this Peanut ELISA Kit, for peanut residues in foodstuffs are required.

Peanut ELISA Kit Test Principle:
The Peanut ELISA Kit is a quantitative test is based on the principle of the enzyme linked immunosorbent assay. An antibody directed against peanut proteins is bound on the surface of a microtiter plate. Peanut containing samples or standards are given into the wells of the microtiter plate on the Peanut ELISA Kit.

Diagnostic Automation Inc offers several popular Food Allergen ELISA Kits: Egg Protein Residue in Food ELISA Kit, Soy Protein Residue Detection in Food ELISA Kit, Lupine ELISA Kit, Mustard ELISA Kit, Soy ELISA Kit, Almond ELISA Kit, Gluten ELISA Kit, Walnut ELISA Kit, and Milk Protein Residue Detection in Food ELISA Kit. For more information on any of these Food ELISA Test Kits please see our website home page, or contact our Customer Service Representative at 818-591-3030.

Product Note:

Due to high risk of cross-contamination all applied instruments like applicator, mortar, glass vials etc. have to be cleaned thoroughly before and after each sample. Peanut proteins adhere very strongly to different surfaces. In certain cases they can resist a common dishwasher cleaning. To identify possible cross-contamination caused by previous extractions Diagnostic Automation, Inc. strongly recommends noting the sequence of the extractions. The limit of detection (LOD) of the DAI Peanut ELISA test kit is 0.1 ppm. The limit of quantification (LOQ) of the DAI Peanut test is 1 ppm. Due to the variety of sample matrices and their influence on the blank, results less than the LOQ should be treated as negative.

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