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Zearalenone Elisa kit


Zearalenone Elisa kit

Category Name Food ELISA kits
Test 96
Principle Quantitative
Specificity N/A
Sensitivity 0.25 ng/mL
Total Time ~200 min
Shelf Life 12-14 Months from the manufacturing date

Item #:                    5148-8   Quantity:               

Zearalenone Elisa kit

Zearalenone ELISA 5148-8 MSDS (03-20-2017).pdf

Zearalenone Elisa kit

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Zearalenone Elisa kit description:

Zearalenone ELISA Test Kit is a quantitative assay based on the principle of the enzyme linked immunosorbent assay. Zearalenone ELISA is a quick, economical, and sensitive method to detect zearalenone in food.

Materials Provided with Zearalenone ELISA Kit:
1. Microtiter plate:12 strips with 8 breakable wells coated zearalenone conjugate
2. Zearalenone Standards (0; 0.5; 2; 20; 200 ng/mL): 5 vials with 1.0 mL each
3. Conjugate (anti-rabbit-IgG-HRP): 15 mL
4. Anti-Zearalenone Antibody (rabbit): 6 mL
5. Substrate Solution (TMB): 15 mL
6. Stop Solution (0.5 M H2SO4): 15 mL
7. Sample diluent (PBS) 2x
8. Washing Solution (PBS + Tween 20): 60 mL as 10x
9. Two plastic foils to cover the strips during the incubation
10. Plastic bag to store unused microtiter strips
11. Instruction Manual

Materials required but not provided:
1. Freshly distilled or deionized water
2. Dispensing system and/or pipette
3. EIA kit Microplate washer
4. EIA kit Microplate Reader with 450nm wavelength

Zearalenone ELISA Test Kit Background Information:
Zearalenone belongs to the group of mycotoxins. Similar to other mycotoxins, zearalenone is a degradation product of the secondary metabolism. It is produced by different moulds of the Fusarium gender. These moulds infect grain and other types of food like peanuts and beans already during their growth. When a considerable amount of zearalenone contaminated feed is taken up by cows, it can also be detected in their milk. Even in beer it could be found. Zearalenone shows a strong estrogen-like activity. Thus zearalenone can cause an enlargement of the uterus, diminution of the ovarian glands and even infertility. Zearalenone is one of the main contaminants of farm products, which can be taken up by humans and animals. In order to protect humans of illnesses caused by zearalenone, a qualitative and quantitative control of threatened food is essential, together with appropriate hygienic measures, which help to prevent the development of zearalenone.

Zearalenone Food Test Principle:
The Diagnostic Automation, Inc. quantitative test is based on the principle of the enzyme linked immunosorbent assay. A zearalenone conjugate is bound on the surface of a microtiter plate. For additional details please refer to the instructions for use.

Diagnostic Automation Inc offers several popular Food Allergen ELISA Kits: Egg Protein Residue in Food ELISA Kit, Soy Protein Residue Detection in Food ELISA Kit, Lupine ELISA Kit, Mustard ELISA Kit, Peanut ELISA Kit, Soy ELISA Kit, Almond ELISA Kit, Gluten ELISA Kit, Walnut ELISA Kit, and Milk Protein Residue Detection in Food ELISA Kit. For more information on any of these Food ELISA Test Kits please see our website home page, or contact our Customer Service Representative at 818-591-3030.

Product Note:

The sensitivity of  Zearalenone ELISA test is 0.25 ng/mL.(based on the standard curve). The recovery of spiked grain samples was determined to 85-95 percent. The intra-assay variation of the zearalenone test was determined to percent.