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HTLV 1/2 ELISA Test

Name

HTLV 1/2 ELISA kit

Full name

Human T-Cell Lymphotropic Viruses Antibody ELISA Test Kit, Export Use Only

Category Name Hepatitis ELISA kits
Test 96
Method ELISA method: Enzyme Linked Immunosorbent Assay
Principle ELISA - Double antigen Sandwich ELISA
Detection Range Qualitative - Positive; Negative control & Cut Off
Sample 50 uL
Specificity 99.98%
Sensitivity 100%
Total Time ~90 min
Shelf Life 12 Months from the manufacturing date

Item #:                    8196-12   Quantity:               

HTLV 1/2 ELISA Test

HTLV_8196-12 (MSDS) (07-08-2016).pdf

HTLV 1/2  ELISA Test

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HTLV 1/2 ELISA Test description:




"EXPORT USE ONLY"
The Diagnostic Automation HTLV ELISA test kit is an enzyme-linked immunosorbent assay (ELISA) for qualitative determination of antibodies to Human T-Cell Lymphotropic Virus types 1 and/or 2 (HTLV-1/2) in human serum or plasma. It is intended for screening of blood donors against HTLV1-2 and as an aid for the diagnosis of clinical conditions related to infection with HTLV-1 and/or HTLV-2.




Materials Provided with HTLV 1/2 Elisa kit:
1. 96 HTLV 1/2 Elisa tests
2. Microwell strips breakable:containing recombinant HTLV 1/2 antigens
3. HTLV 1/2 Elisa test Negative Control
4. HTLV 1/2 Elisa test Positive Control
5. HRP-Conjugated HTLV 1/2 Antigens
6. Stock Wash Buffer 20 x
7. Chromogen Solution A HTLV 1/2 EIA Test
8. Chromogen Solution B HTLV 1/2 EIA Test
9. Stop Solution
10. Plastic sealable Bag, 1unit
11. Cardboard Plate Cover, 2sheets
12. Package Insert

Materials & Instrumentations Required, not provided:
1. Freshly distilled or deionized water
2. Dispensing system and/or pipette & tips
3. Dry incubator or water bath
4. Microshaker
5. EIA kit Microplate Washer
6. EIA kit Microplate Reader at 450nm wavelength





HTLV 1/2 Background Information:
The (HTLV) human T-cell lymphotropic viruses is a member of the family of Retroviridae, consisting of enveloped double stranded RNA viruses and genetically not related to HIV1&2; however, they have similar routes of transmission and can have extremely long period of latency prior to manifestation of disease. HTLV type 1 was reported in 1980 as the first retrovirus shown to be pathogenic to humans. The virus preferentially infects CD4 + lymphocytes while the infections of CD8 + T lymphocytes are rare. In contrast to HTLV 1, HTLV type 2 can infect all type of lymphocytes as well as the macrophages. HTLV 1&2 is transmitted transplacentally, paternally, by infected blood and through sexual contacts. The diseases associated with HTLV infection are usually classified as malignant or nonmalignant. HTLV 1 is endemic in the Caribbean, southern Japan, in the US and many other scattered population through the world. HTLV 2 is endemic in some North American Indian tribes but, is detected mostly in intravenous drug users and their sexual partners.




HTLV 1/2 Elisa Test Principle:
The HTLV ELISA kit is one step incubation, antigen a sandwich enzyme immunoassay (ELISA) method, which uses polystyrene microwell strips pre-coated with recombinant HTLV antigens expressed in E.coli. Patient serum/plasma sample is incubated in the microwells together with second recombinant HTLV antigens conjugated to horseradish peroxidase (HRP-Conjugate). The pre-coated antigens express the same epitopes as the HRP-Conjugate antigens, but are expressed in different hosts. In case of presence of anti-HTLV in sample, the pre-coated and HRP-conjugated antigens will be bound to the two variable domains of the antibody and during incubation, the specific antigen-antibody immunocomplex is captured on the solid phase. After washing to remove sample and unbound HRP-Conjugate, Chromogen solutions containing tetramethylbenzidine (TMB) and urea peroxide are added to the wells. In presence of the antigen-antibody-antigen(HRP) a sandwich complex, the colorless Chromogens are hydrolyzed by the bound HRP-Conjugate to a blue-colored product. The blue color turns yellow after stopping the reaction with sulfuric acid. The amount of color intensity can be measured and is proportional to the amount of antibody captured in the wells, and to the sample respectively. Wells containing samples negative for anti-HTLV remain colorless.

Diagnostic Automation Inc. also provides other Test Kits. For more information about ELISA Kits, Rapid Tests, IFA Kits, CLIA Test Kits, or Serology tests, please see our website home page, or contact our Customer Service Representative at 818-591-3030.


* Not to be sold, shipped or distributed in The United States.







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