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Lyme Disease IgG/IgM ELISA Test


Lyme Disease IgG/IgM ELISA kit

Full name

Human Lyme Disease IgG/IgM ELISA Test kit

Category Name Infectious Disease ELISA kits
Test 96
Method ELISA: Enzyme Linked Immunosorbent Assay
Principle Sandwich Complex
Detection Range Qualitative Positive; Negative control & Cut off
Sample 10 ul serum
Total Time ~60 min
Shelf Life 12 Months from the manufacturing date

Item #:                    1423-P2   Quantity:               

Lyme Disease IgG/IgM ELISA Test

Lyme Disease IgG/IgM ELISA Test

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Lyme Disease IgG/IgM ELISA Test description:

Lyme Disease ELISA Test, known as Lyme Disease IgG/IgM ELISA Test kit, is a B. burgdorferi ELISA test system designed to detect both IgM and IgG class antibodies to Borrelia burgdorferi in human sera.

Materials provided with B burgdorferi IgG/IgM elisa kit:
1. Plate: wells coated with inactivated B burgdorferi (B31 strain) antigen
2. HRP-Conjugate goat anti-human IgM / IgG
3. Positive Control: Human Serum
4. Calibrator: Human Serum
5. Negative Control: Human Serum
6. Absorbent Solution: goat anti-human
7. Sample Diluent
8. TMB
9. Stop Solution
10. Wash Buffer Concentrate 10X
11. Package Insert

Materials & Instrumentations Required, not provided:
1. Freshly distilled or deionized water
2. Dispensing system and/or pipette & tips
3. Dry incubator or water bath
4. Microshaker for dissolving and mixing conjugate with samples
5. EIA kit Microplate Washer
6. EIA kit Microplate Reader at 450nm wavelength

Lyme Disease IgG/IgM elisa kit Background Information:
Borrelia burgdorferi is a spirochete that causes Lyme disease. The organism is transmitted by ticks of the genus Ixodes. Patients with early infection produce IgM antibodies during the first few weeks after onset of erythema migrans (EM) and produce IgG antibodies more slowly. Although IgM only may be detected during the first month after onset of illness, the majority of patients develop IgG antibodies within one month. Both IgG and IgM antibodies can remain detectable for years. Serological testing methods for antibodies to B. burgdorferi include indirect fluorescent antibody (IFA) staining, immunoblotting, and enzyme immunoassay (EIA). B. burgdorferi is antigenically complex with strains that vary considerably. Early antibody responses often are to flagellum which has cross reactive components. Patients in early stages of infection may not produce detectable levels of antibody. Also, early antibiotic therapy after EM may diminish or abrogate good antibody response. Some patients may never generate detectable antibody levels. Thus, serological tests for antibodies to B. burgdorferi are known to have low sensitivity and specificity and because of such inaccuracy, these tests cannot be relied upon for establishing a diagnosis of Lyme disease.

Lyme Disease IgG/IgM ELISA Test Principle:
Borrelia burgdorferi ELISA test kit is designed to detect IgG/IgM class antibodies to B. burgdorferi in human sera. Wells of plastic microwell strips are sensitized by passive absorption with B. burgdorferi antigen. Use of the Lyme disease test kit procedure involves three incubation steps. For additional details please refer to the instructions for use.
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Diagnostic Automation Inc.provides two other Lyme Disease ELISA Test Kits:
1. Lyme Disease IgG ELISA Test Kit which is an ELISA test system designed to detect IgG antibodies to B. burgdorferi in human sera.
2. Lyme Disease IgM ELISA Test Kit which is designed to detect IgM class antibodies to B. burgdorferi in human sera.
For more information about these Lyme Disease ELISA Test Kits, Rapid Tests, IFA Kits, CLIA Test Kits, or Serology tests, please see our website home page, or contact our Customer Service Representatives at 818-591-3030 .

Product Note:

This Lyme Disease IgG/ IgM ELISA Test should only be used for patients with signs and symptoms that are consistent with Lyme disease.