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Human HBcAb ELISA Test Kit, Export Use Only

Category Name Infectious Disease ELISA kits
Test 96
Method ELISA method: Enzyme Linked Immunosorbent Assay
Principle Competitive ELISA
Detection Range Qualitative Positive; Negative control & Cut off
Sample 10ul Serum
Total Time ~ 75 min
Shelf Life 12 Months from the manufacturing date

Item #:                    1704-P1   Quantity:               




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HBcAb ELISA kit description:

"Export Use Only"
The HBcAb ELISA test kit is an enzyme-linked immunosorbent assay for the qualitative identification of antibodies to hepatitis B antigen (HBcAg) in human serum/plasma.

Materials included with HBcAb ELISA test kit:
1. Microwell Plate: 96 wells coated with HBcAg
2. Negative Control: Protein-stabilized buffer non-reactive for anti-HBc
3. Positive Control: Purified anti-HBc diluted in protein stabilized buffer
4. HRP-Conjugated Reagent: HRP-conjugated anti- HBc
5. Stock Wash Buffer: PH 7.4, 20x PBS
6. Chromogen Solution A: Urea peroxide solution
7. Chromogen Solution B: TMB solution
8. Stop Solution: Diluted sulfuric acid solution
9. Plastic Sealable Bag
10. Cardboard Plate Cover
11. Package Insert

Materials required but not provided:
1. Freshly distilled or deionized water
2. Dispensing system and/or pipette
3. Microshaker for dissolving and mixing conjugate with samples
4. EIA kit Microplate washer
5. EIA kit Microplate Reader with 450nm or dual wavelength 450 & 630nm

HBcAb ELISA test Background Information:
As part of the Hepadnaviridae family, HBV is an enveloped, double-stranded DNA virus that is a primary cause of hepatitis transmission through blood. The effects of HBV infection range anywhere from mild to severe hepatitis, which includes chronic liver problems, such as carcinoma and cirrhosis. In order to classify hepatitis B infection, the serological markers need to be identified during the three phases of the infection - incubation, acute, and convalescent. The main component of the virus is Hepatitis B core antigen (HBcAg). This core antigen is comprised of a single polypeptide of approximately 17kD that is discharged upon disaggregation of the core particles. At least one immunological determinant is present in the antigen.

HBcAb ELISA test Principle:
The system of the HBcAb ELISA test is founded on the solid phase, one-step incubation competitive principle. When anti-HBc is present, it competes with monoclonal anti-HBc conjugated to horseradish peroxidase (HRP-Conjugate) for a fixed amount of purified HBcAg pre-coated in the wells. For additional details please refer to the instructions for use.

For more information about ELISA Kits, Rapid Tests, IFA Kits, CLIA Test Kits, or Serology tests, please see our website home page, or contact our Customer Service Representative at 818-591-3030.

* Not to be sold, shipped or distributed in The United States.