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Legionella Urine ELISA kit


Legionella ELISA Test

Full name

Human Legionella ELISA Test Kit - Urinary Antigen (Urine)

Category Name Parasitology ELISA kits
Test 96
Method ELISA method: Enzyme Linked Immunosorbent Assay
Principle ELISA principle- Sandwhich; Antibody Coated Plate
Detection Range Qualitative elisa assay- Positive, Negative Controls
Sample 1gm Stool Sample
Specificity 99.2%
Sensitivity 100%
Total Time ~45 min
Shelf Life 12 Months from the manufacturing date

Item #:                    8305-3   Quantity:               

Legionella Urine ELISA kit


Legionella Urine ELISA kit

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Legionella Urine ELISA kit description:

This Legionella Urinary Antigen test is intended as an adjunct to culture for the presumptive diagnosis of past or current Legionnaires disease by qualitative detection of Legionella pneumophilia antigen in human urine.

Material Provided with Legionella Urinary ELISA kit:
1.Plate: Microwells containing purified anti- Legionella pneumophilia antibodies.
2.Enzyme Conjugate: One (1) bottle containing 11 ml of anti- Legionella pneumophilia antibodies conjugated to horseradish peroxidase with red dye and Thimerosal.
3.Positive Control: One (1) vial containing 2 ml of diluted Legionella pneumophilia antigen in buffer with Thimerosal.
4.Negative Control: One (1) vial containing 2 ml of buffer with Thimerosal.
5.Chromogen: One (1) bottle containing 11 ml of tetramethylbenzidine (TMB) and peroxide.
6.Wash Concentrate 20X: One (1) bottle containing 25 ml of concentrated buffer with surfactant and Thimerosal.
7.Stop Solution: One (1) bottle containing 11 ml of 5% phosphoric acid solution.

Materials Required, not Provided:
1. Precision pipettes
2. Distilled or deionized water
3. EIA kit Microplate Washer
4. EIA kit Microplate Reader with a 450 nm/620 - 650 nm filter

Legionella Urinary ELISA kit Background Information
Legionella pneumophilia was first isolated and characterized in 1976 after a major outbreak of pneumonia at an American Legion convention in Philadelphia, Pennsylvania; thus the name Legionnaires Disease. Traditional laboratory methods for the detection of pneumonia caused by Legionella pneumophilia infection requires an adequate respiratory specimen (e.g. sputum, bronchial washings, transtracheal aspirate, lung biopsy) or paired serum specimens (acute and convalescent) for accurate diagnosis. Accurate diagnosis of Legionella pneumophilia using these methods depends upon proper specimen collection and the level of technical expertise available. Approximately 80% of Legionella pneumonia patients excrete soluble Legionella antigen in their urine. This presents the opportunity for rapid detection of Legionella Urine Antigen (LUA) in a urine specimen.

Legionella Urinary ELISA Test Principle:
The Legionella Urine Antigen ELISA is designed to detect Legionella antigen in Human Urine sample. This Legionella urine test is a double antibody (sandwich) ELISA using an anti-Legionella pneumophilia antibody to capture the antigen from the urine. A second antibody, conjugated to peroxidase (HRP), is then added which binds to the complex. This reaction is visualized by the addition of the chromogen TMB. The resulting blue color development indicates the presence of Legionella pneumophilia antigens being bound by the anti- Legionella pneumophilia antibodies.

Notable features of the Parasitology ELISA Kits:
User-friendly directions and explanation of test procedures
Simple and safe reagent preparation
Clear instructions on specimen collection
Comprehensive package of required materials
Explicit quality control and storage guidelines
Reliable and easy-to-read test results

Product inserts for most Parasitology ELISA kits follow a similar method. See the Legionella Urinary ELISA Kit product insert for details on preparation, procedures, quality control, and test result interpretation.

Diagnostic Automation also provides other Parasitology ELISA Kits. For more information about ELISA Kits or Rapid Tests, IFA Kits, CLIA Test Kits, or Serology tests, please see our website home page, or contact our Customer Service Representative at 818-591-3030.

Product Note:

This Legionella ELISA kit is a urinary ELISA test and urine is the preferred specimen for collection, transport, and detection in all phases of the disease. Specific soluble Legionella antigen is present in the urine of patients with Legionnaires disease.

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